Ribospin™ vRD plus! provides a convenient method for isolation of RNA and DNA from cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples. Ribospin™ vRD plus! procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA and DNA isolation, instead of conventional alcohol precipitation or phenol/chloroform extraction. Ribospin™ vRD plus! buffer system provides the effective binding condition of RNA and DNA to glassfiber membrane through mix with lysis and binding buffers. And then the impurities on the membrane are washed away by two different wash buffers. At last, pure RNA and DNA are eluted by nuclease-free water. Whole procedure may take only 15 minutes and the eluate is suitable for PCR, RT-PCR, or any downstream application without further manipulation.
Ribospin™ vRD plus! procedure should be performed at room temperature. The eluate should be treated with care because RNA is very sensitive to contaminants, such as RNases, often found on general labware and dust. To ensure RNA-stability, it is recommended to store at 4°C for immediate analysis or to freeze at -70°C for long-term storage.